中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

HEV核酸、抗原、抗体对HEV感染的诊断价值

李晓鹤 王江华 季颖 刘艳 朱凌 贾玉缘 王震宇 史羿君 张海莹 饶慧瑛

引用本文:
Citation:

HEV核酸、抗原、抗体对HEV感染的诊断价值

DOI: 10.3969/j.issn.1001-5256.2020.01.022
基金项目: 

国家自然科学基金青年基金(81802047,81870406); 北京市自然科学基金(7182174); “十三五”期间国家传染病防治科技重大专项(2017ZX10202202); 2016年北京大学人民医院研究与发展基金(RDY2016-13); 

详细信息
  • 中图分类号: R512.6

Value of hepatitis E virus nucleic acid,antigen,and antibodies in the diagnosis of hepatitis E virus infection

Research funding: 

 

  • 摘要:

    目的探讨HEV核酸(HEV RNA)、HEV抗原(HEV Ag)、HEV抗体(HEV IgM抗体和HEV IgG抗体)在临床诊断HEV感染中的作用。方法收集在北京大学人民医院进行HEV IgM抗体和HEV IgG抗体检测的13 992例标本,其中1924例HEV IgM抗体或HEV IgG抗体阳性。分别采用荧光PCR法进行HEV RNA和基因型检测,酶联免疫吸附试验方法进行HEV Ag、HEV IgM抗体和HEV IgG抗体检测,并检测ALT、AST、TBil和DBil水平。计量资料两组间比较采用Mann-Whitney U检验。结果 1924例标本中HEV IgM抗体阳性152例(7. 9%),HEV IgG抗体阳性1897例(98. 6%),HEV RNA阳性62例(3. 2%),HEV Ag阳性55例(2. 9%)。HEV IgM抗体阳性组中HEV RNA阳性率为40. 8%(62/152),HEV Ag阳性率为36. 2%(55/152)。HEV RNA阳性组中HEV Ag阳性率为88. 7%(55/62)。HEV RNA阳性组(n=62) ALT、AST、TBil、DBil水...

     

  • [1] NIMGAONKAR I,DING Q,SCHWARTZ RE,et al. Hepatitis E virus:Advances and challenges[J]. Nat Rev Gastroenterol Hepatol,2018,15(2):96-110.
    [2] CUI T,ZHANG X,WANG Q,et al. Disease burden concerning hepatitis E-infected inpatients in Jiangsu province,China[J]. Vaccine,2019.[Epub ahead of print]
    [3] LIU ZQ,ZUO JL,YAN Q,et al. Epidemiologic and spatiotemporal characteristics of hepatitis E in China 2004-2014[J]. Chin J Epidemiol,2017,38(10):1380-1385.(in Chinese)刘振球,左佳鹭,严琼,等.我国2004-2014年戊型肝炎流行的时空特征及趋势分析[J].中华流行病学杂志,2017,38(10):1380-1385.
    [4] ZHOU YH,ZHUANG H. Recent advances in the epidemiological research on hepatitis E in China[J]. Chin J Epidemiol,2010,31(12):1414-1416.(in Chinese)周乙华,庄辉.中国戊型肝炎流行病学研究进展[J].中华流行病学杂志,2010,31(12):1414-1416.
    [5] AGGARWAL R. Hepatitis E:Clinical presentation in disease-endemic areas and diagnosis[J]. Semin Liver Dis,2013,33(1):30-40.
    [6] PETRIK J,LOZANO M,SEED CR,et al. Hepatitis E[J]. Vox Sang,2016,110(1):93-130.
    [7] KAMAR N,DALTON HR,ABRAVANEL F,et al. Hepatitis E virus infection[J]. Clin Microbiol Rev,2014,27(1):116-138.
    [8] SHUAI JB,LI LH,LI AY,et al. Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China[J]. J Zhejiang Univ Sci B,2017,18(6):549-554.
    [9] PAS SD,STREEFKERK RH,PRONK M,et al. Diagnostic performance of selected commercial HEV IgM and IgG ELISAs for immunocompromised and immunocompetent patients[J]. J Clin Virol,2013,58(4):629-634.
    [10] WU WC,SU CW,YANG JY,et al. Application of serologic assays for diagnosing acute Hepatitis E in national surveillance of a nonendemic area[J]. J Med Virol,2014,86(4):720-728.
    [11] NORDER H,KARLSSON M,MELLGREN A,et al. Diagnostic performance of five assays for anti-hepatitis E virus IgG and IgM in a large cohort study[J]. J Clin Microbiol,2016,54(3):549-555.
    [12] HYAMS C,MABAYOJE DA,COPPING R,et al. Serological cross reactivity to CMV and EBV causes problems in the diagnosis of acute hepatitis E virus infection[J]. J Med Virol,2014,86(3):478-483.
    [13] WANG Y,LIU H,LIU S,et al. Incidence,predictors and prognosis of genotype 4 hepatitis E related liver failure:A tertiary nested case-control study[J]. Liver Int,2019.[Epub ahead of print]
    [14] WANG L,LIU L,WEI Y,et al. Clinical and virological profiling of sporadic hepatitis E virus infection in China[J]. J Infect,2016,73(3):271-279.
  • 加载中
计量
  • 文章访问数:  1328
  • HTML全文浏览量:  32
  • PDF下载量:  275
  • 被引次数: 0
出版历程
  • 收稿日期:  2019-08-09
  • 出版日期:  2020-01-20
  • 分享
  • 用微信扫码二维码

    分享至好友和朋友圈

目录

    /

    返回文章
    返回