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非酒精性脂肪性肝炎大鼠模型的建立及其胰腺、肾脏的组织学病理变化

宋清莲 谭玉娥 刘京京 袁贝贝 李敏 贺礼琴 戴光荣

引用本文:
Citation:

非酒精性脂肪性肝炎大鼠模型的建立及其胰腺、肾脏的组织学病理变化

DOI: 10.3969/j.issn.1001-5256.2020.01.031
基金项目: 

陕西省社会发展科技攻关项目(2016SF-120); 

详细信息
  • 中图分类号: R575;R-332

Establishment of a rat model of nonalcoholic steatohepatitis and histopathological changes of the pancreas and the kidney

Research funding: 

 

  • 摘要: 目的探讨非酒精性脂肪性肝炎大鼠模型肝外脏器胰腺、肾脏的组织学病理变化情况。方法将20只SD大鼠中的6只首先按完全随机配对分配到观察组(B组),将剩下的14只按体质量配对成7个区组,最后将每个区组中的1只大鼠按随机数字表分配到实验组(C组),其余7只为对照组(A组)。B组和C组给予高脂饮食,A组给予普通饮食。B组于实验第4、8、12周各处死2只,提取肝脏组织行HE染色,显微镜下观察肝脏病理切片是否达到脂肪性肝炎,第12周末建模成功(提示C组建模成功)。处死A组和C组全部大鼠,分别检测血清学指标ALT、AST、GGT、TG、TC、LDL、GLU、INS、HOMA-IR,取肝脏组织行HE染色;胰腺组织行HE染色观察大鼠胰腺腺泡和胰岛变化情况;肾脏组织行PAS染色,免疫组织化学desmin蛋白染色观察肾小球和足细胞desmin表达情况。计量资料2组间比较采用t检验。结果 C组大鼠病理评分及血清ALT、GGT、LDL、TG、TC、GLU、INS、HOMAIR水平均高于A组(t值分别为4. 67、2. 83、2. 34、4. 58、4. 78、3. 00、2. 97、3. 80、4. 10,P值均...

     

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  • 收稿日期:  2019-09-09
  • 出版日期:  2020-01-20
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