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草苁蓉环烯醚萜苷(IGBR)对TGF-β1诱导的HepG2细胞上皮间质转化模型的影响

金爱花 朱洁波 尹学哲 全吉淑

引用本文:
Citation:

草苁蓉环烯醚萜苷(IGBR)对TGF-β1诱导的HepG2细胞上皮间质转化模型的影响

DOI: 10.12449/JCH240617
基金项目: 

国家自然科学基金 (82060113);

国家自然科学基金 (81760659);

吉林省教育厅项目 (JJKH20210578KJ)

利益冲突声明:本文不存在任何利益冲突。
作者贡献声明:金爱花负责课题设计,资料分析,撰写论文,参与实验;朱洁波参与数据分析与实验;尹学哲参与数据分析与修改论文;全吉淑负责拟定写作思路,指导撰写文章并最后定稿。
详细信息
    通信作者:

    全吉淑, quanjs@ybu.edu.cn (ORCID: 0000-0002-6466-5787)

Effect of iridoid glycosides from Boschniakia rossica on epithelial-mesenchymal transition of HepG2 cells induced by transforming growth factor-beta 1

Research funding: 

National Natural Science Foundation of China (82060113);

National Natural Science Foundation of China (81760659);

Jilin Province Education Department project (JJKH20210578KJ)

More Information
    Corresponding author: QUAN Jishu, quanjs@ybu.edu.cn (ORCID: 0000-0002-6466-5787)
  • 摘要:   目的  研究草苁蓉环烯醚萜苷(IGBR)对TGF-β1诱导肝癌HepG2细胞上皮间质转化(EMT)的影响作用。  方法  用10 μg/L TGF-β1诱导HepG2肝癌细胞株构建肝癌细胞EMT模型。实验分为对照组、模型组与IGBR组3组,对照组用无血清DMEM处理,模型组用10 μg/L TGF-β1处理,IGBR组用10 μg/L TGF-β1和500 mg/L IGBR联合处理,培养48 h。利用细胞黏附实验、划痕愈合实验和Transwell小室实验观察细胞迁移和侵袭能力。RT-PCR法和Western Blot法检测细胞中E-钙黏蛋白、N-钙黏蛋白、波形蛋白的mRNA和蛋白表达,Western Blot法检测Slug、Twist1、ZEB1、p-STAT3、STAT3的蛋白表达。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验;两组间比较采用成组t检验。  结果  TGF-β1诱导后,模型组HepG2细胞呈现长梭形改变;与模型组比较,IGBR组细胞黏附率降低,抑制细胞迁移、侵袭能力(P值均<0.05),E-钙黏蛋白的mRNA表达和蛋白表达均增高(P值均<0.05),N-钙黏蛋白和波形蛋白的mRNA表达和蛋白表达均降低(P值均<0.05),Slug、Twist1、ZEB1蛋白表达和p-STAT3蛋白表达均降低(P值均<0.05)。  结论  IGBR可抑制TGF-β1诱导的HepG2细胞EMT过程,从而减弱HepG2细胞黏附力和细胞迁移、侵袭能力,上调E-钙黏蛋白,下调N-钙黏蛋白和波形蛋白,上调Slug、Twist1、ZEB1、STAT3的蛋白表达,其作用可能通过抑制STAT3通路下调Slug、Twist1、ZEB1等EMT转录因子来实现。

     

  • Figure  .  The EMT model of HepG2 cells was induced by TGF-β1

    图  2  IGBR对HepG2细胞形态的影响(×100)

    注: a,对照组;b,模型组;c,IGBR组。

    Figure  2.  Effect of IGBR on HepG 2 cell morphology(×100)

    图  3  IGBR对HepG2细胞迁移能力的影响(×40)

    Figure  3.  Effect of IGBR on the migratory ability of HepG 2 cells(×40)

    图  4  IGBR对HepG2细胞侵袭能力的影响(×100)

    注: a,对照组;b,模型组;c,IGBR组。

    Figure  4.  Effect of IGBR on the invasive ability of HepG 2 cells(×100)

    图  5  IGBR对HepG2细胞E-钙黏蛋白、N-钙黏蛋白和波形蛋白表达的影响

    注: a,RT-PCR结果;b,Western Blot结果。

    Figure  5.  Effect of IGBR on the expression of E-cadherin, N-cadherin, and vimentin in HepG 2 cells

    图  6  IGBR对HepG2细胞EMT相关转录因子表达的影响

    Figure  6.  Effect of IGBR on the expression of EMT-associated transcription factors in HepG 2 cells

    图  7  IGBR对HepG2细胞STAT3蛋白表达的影响

    Figure  7.  Effect of IGBR on STAT 3 protein expression in HepG 2 cells

    表  1  HepG2细胞EMT模型中E-钙黏蛋白、N-钙黏蛋白和波形蛋白表达的比较

    Table  1.   Comparison of E-cadherin, N-cadherin, and vimentin expression in the EMT model of HepG2 cells

    组别 mRNA 蛋白
    E-钙黏蛋白 N-钙黏蛋白 波形蛋白 E-钙黏蛋白 N-钙黏蛋白 波形蛋白
    对照组 1.41±0.11 0.25±0.06 0.36±0.08 1.40±0.13 0.42±0.06 0.36±0.12
    模型组 0.26±0.09 1.45±0.14 0.89±0.10 0.31±0.09 1.63±0.14 2.10±0.10
    t 14.015 13.646 7.168 11.940 13.759 19.294
    P 0.000 2 0.000 2 0.002 0 0.000 3 0.000 2 <0.000 1
    下载: 导出CSV

    表  2  IGBR对HepG2细胞E-钙黏蛋白、N-钙黏蛋白和波形蛋白表达的比较

    Table  2.   Comparison of IGBR on E-cadherin, N-cadheractin, and vimentin expression in HepG 2 cells

    组别 mRNA 蛋白
    E-钙黏蛋白 N-钙黏蛋白 波形蛋白 E-钙黏蛋白 N-钙黏蛋白 波形蛋白
    对照组 0.80±0.05 0.17±0.06 0.14±0.04 1.45±0.05 0.12±0.06 0.14±0.05
    模型组 0.20±0.091) 0.78±0.061) 1.20±0.101) 0.25±0.031) 1.08±0.091) 1.37±0.101)
    IGBR组 0.42±0.072) 0.58±0.032) 0.65±0.092) 0.08±0.102) 0.70±0.122) 1.10±0.142)
    F 53.50 107.40 128.40 242.40 69.01 127.90
    P 0.000 1 <0.000 1 <0.000 1 <0.000 1 <0.000 1 <0.000 1
    注:与对照组相比,1) P<0.05;与模型组相比,2) P<0.05。
    下载: 导出CSV
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